pCS2+ Zf ctnna1L344P
(Plasmid #185407)

• Purpose: Zebrafish ctnna1 bearing a point mutation in the vinculin binding site. For in vitro transcription (SP6) or expression through CMV
• Depositing Lab: Nicolas David
• Publication: Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(22)00329-X. doi: 10.1016/j.devcel.2022.05.001.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4095
• Total vector size (bp): 6822
• Vector type: Mammalian Expression ; In vitro synthesis of mRNA

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ctnna1
• Alt name: alpha-catenin
• Species: D. rerio (zebrafish)
• Insert Size (bp): 2700
• Mutation: changed Leucine 344 to Proline
• Entrez Gene: ctnna1 (a.k.a. ctnna)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Ordered from Twist Bioscience

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2021.04.26.441407v2 for bioRxiv preprint.