RS17055_pMQ30_KO_Kan
(Plasmid
#185398)
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PurposeNon-replicating vector used to create markerless deletion of RR42_RS17055 in C. basilensis 4G11
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 185398 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepMQ30
- Backbone size w/o insert (bp) 6918
- Total vector size (bp) 7159
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Modifications to backboneinserted homology regions upstream and downstream to the RR42_RS17055 gene of C.basilensis 4G11. Replaced original GmR gentamycin resistance marker with nptII kanamycin resistance marker.
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameRR42_RS17055 upstream flanking homology region
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Insert Size (bp)497
Gene/Insert 2
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Gene/Insert nameRR42_RS17055 downstream flanking homology region
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Insert Size (bp)500
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
C. basilensis genomic regions from genomic DNA of C. basilensis 4G11: doi: 10.1128/genomeA.00322-15.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RS17055_pMQ30_KO_Kan was a gift from Adam Arkin (Addgene plasmid # 185398 ; http://n2t.net/addgene:185398 ; RRID:Addgene_185398) -
For your References section:
Eliminating genes for a two-component system increases PHB productivity in Cupriavidus basilensis 4G11 under PHB suppressing, nonstress conditions. Sander K, Abel AJ, Friedline S, Sharpless W, Skerker J, Deutschbauer A, Clark DS, Arkin AP. Biotechnol Bioeng. 2024 Jan;121(1):139-156. doi: 10.1002/bit.28532. Epub 2023 Aug 28. 10.1002/bit.28532 PubMed 37638652
Map uploaded by the depositor.
