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RS35075_pMQ30_KO_Kan
(Plasmid #185390)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185390 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMQ30
  • Backbone size w/o insert (bp) 6918
  • Total vector size (bp) 7159
  • Modifications to backbone
    inserted homology regions upstream and downstream to the RR42_RS35075 gene of C.basilensis 4G11. Replaced original GmR gentamycin resistance marker with nptII kanamycin resistance marker.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    RR42_RS35075 upstream flanking homology region
  • Insert Size (bp)
    500

Gene/Insert 2

  • Gene/Insert name
    RR42_RS35075 downstream flanking homology region
  • Insert Size (bp)
    500

Gene/Insert 3

  • Gene/Insert name
    nptII kanamycin resistance marker
  • Insert Size (bp)
    795

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

C. basilensis genomic regions from genomic DNA of C. basilensis 4G11: doi: 10.1128/genomeA.00322-15.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RS35075_pMQ30_KO_Kan was a gift from Adam Arkin (Addgene plasmid # 185390 ; http://n2t.net/addgene:185390 ; RRID:Addgene_185390)

  • For your References section:

    Eliminating genes for a two-component system increases PHB productivity in Cupriavidus basilensis 4G11 under PHB suppressing, nonstress conditions. Sander K, Abel AJ, Friedline S, Sharpless W, Skerker J, Deutschbauer A, Clark DS, Arkin AP. Biotechnol Bioeng. 2024 Jan;121(1):139-156. doi: 10.1002/bit.28532. Epub 2023 Aug 28. 10.1002/bit.28532 PubMed 37638652
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