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Addgene

pcDNA5-FRT-TO-hPKAalpha-Flag
(Plasmid #185380)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185380 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5-FRT/TO-Hygro
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin, only when integrated into FlpIn FRT site

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PRKACA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1053
  • Mutation
    WT
  • Entrez Gene
    PRKACA (a.k.a. CAFD1, PKACA, PPNAD4)
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5-FRT-TO-hPKAalpha-Flag was a gift from Ayaz Najafov (Addgene plasmid # 185380 ; http://n2t.net/addgene:185380 ; RRID:Addgene_185380)
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