pX459-puro-hGSDMD
(Plasmid #185377)

• Purpose: For mammalian expression of guide RNA: CGCGCCAGACGCGCCACCCT that targets human GSDMD (Gasdermin D)
• Depositing Lab: Ayaz Najafov
• Publication: Najafov lab plasmids (unpublished)

Backbone

• Vector backbone: pX459-Puro
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GSDMD
• gRNA/shRNA sequence: guide RNA: CGCGCCAGACGCGCCACCCT
• Species: H. sapiens (human)
• Insert Size (bp): 26
• Mutation: WT
• Entrez Gene: GSDMD (a.k.a. DF5L, DFNA5L, FKSG10, GSDMDC1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BbsI (destroyed during cloning)
• 3′ cloning site: BbsI (destroyed during cloning)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry