pLKO-Tet-puro-hARAF-shRNA-1
(Plasmid #185370)

• Purpose: For tetracycline-inducible mammalian expression of shRNA: GCCGTGACCAGATTATCTTTA that targets human ARAF
• Depositing Lab: Ayaz Najafov
• Publication: Najafov lab plasmids (unpublished)

Backbone

• Vector backbone: Tet-pLKO-puro
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ARAF
• gRNA/shRNA sequence: shRNA: GCCGTGACCAGATTATCTTTA
• Species: H. sapiens (human)
• Insert Size (bp): 58
• Mutation: WT
• Entrez Gene: ARAF (a.k.a. A-RAF, ARAF1, PKS2, RAFA1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry