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Addgene

pX459-puro-hARAF-1
(Plasmid #185367)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185367 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pX459-Puro
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ARAF
  • gRNA/shRNA sequence
    guide RNA: caccgTGGTCTACCGACTCATCAAG
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    26
  • Mutation
    WT
  • Entrez Gene
    ARAF (a.k.a. A-RAF, ARAF1, PKS2, RAFA1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (destroyed during cloning)
  • 3′ cloning site BbsI (destroyed during cloning)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pX459-puro-hARAF-1 was a gift from Ayaz Najafov (Addgene plasmid # 185367 ; http://n2t.net/addgene:185367 ; RRID:Addgene_185367)
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