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Addgene

pETDUET-GST-AUP1 379-410:Ube2G2
(Plasmid #185335)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185335 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETDUET
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AUP1 aa 379-410, Ube2G2
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Entrez Gene
    AUP1
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI, NdeI (unknown if destroyed)
  • 3′ cloning site HindII, XhoI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Encodes G2BR of AUP1 and UBE2G2 (from mouse cDNA - amino acid sequence identical to human).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETDUET-GST-AUP1 379-410:Ube2G2 was a gift from Allan Weissman (Addgene plasmid # 185335 ; http://n2t.net/addgene:185335 ; RRID:Addgene_185335)

  • For your References section:

    A structurally conserved site in AUP1 binds the E2 enzyme UBE2G2 and is essential for ER-associated degradation. Smith CE, Tsai YC, Liang YH, Khago D, Mariano J, Li J, Tarasov SG, Gergel E, Tsai B, Villaneuva M, Clapp ME, Magidson V, Chari R, Byrd RA, Ji X, Weissman AM. PLoS Biol. 2021 Dec 8;19(12):e3001474. doi: 10.1371/journal.pbio.3001474. eCollection 2021 Dec. 10.1371/journal.pbio.3001474 PubMed 34879065
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