ApoE3_D125I_mCh-SspB (pBS1145)
(Plasmid #185327)

• Purpose: For the mammalian expression of the human protein ApoE3_D125I attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation
• Depositing Lab: Pamela Silver
• Publication: Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1302. doi: 10.1021/acssynbio.1c00572. Epub 2022 Feb 18.

Backbone

• Vector backbone: YK 02 mCh SspB
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ApoE3_D125I
• Species: H. sapiens (human)
• Insert Size (bp): 954
• Mutation: D125I

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pBS1145.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid encodes a protein fused to SspB. SspB will bind to iLID upon exposure to blue light. We used it to test for light inducible condensate formations using a scaffold protein (FTH1) bound to iLID which was expressed off a separate plasmid (see pBS1042 for the scaffold protein).