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ApoE3_D125I_47-300_mCh-2xFKBP (pBS1114)
(Plasmid #185309)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185309 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    rt44a_nCoV-N-mEGFP-2xFKBP-HA pcDNA4_TO
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ApoE3_D125I_47-300
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    954
  • Mutation
    D125I

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid encodes a protein fused to 2xFKBP. FKBP will dimerize upon exposure to a dimerization agent. We used it to test for chemically inducible condensate formation

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ApoE3_D125I_47-300_mCh-2xFKBP (pBS1114) was a gift from Pamela Silver (Addgene plasmid # 185309 ; http://n2t.net/addgene:185309 ; RRID:Addgene_185309)

  • For your References section:

    Natural and Designed Proteins Inspired by Extremotolerant Organisms Can Form Condensates and Attenuate Apoptosis in Human Cells. Veling MT, Nguyen DT, Thadani NN, Oster ME, Rollins NJ, Brock KP, Bethel NP, Lim S, Baker D, Way JC, Marks DS, Chang RL, Silver PA. ACS Synth Biol. 2022 Mar 18;11(3):1292-1302. doi: 10.1021/acssynbio.1c00572. Epub 2022 Feb 18. 10.1021/acssynbio.1c00572 PubMed 35176859
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