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Addgene

LLPS1-iLID::EGFP::FTH1 (pBS1042)
(Plasmid #185289)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185289 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    YK 02
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    iLID::eGFP::FTH1
  • Species
    De novo

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is a scaffold protein that presents iLID for other proteins to bind upon exposure to light. It was inspired by Addgene plasmid number 122149, simply cloned into the backbone we used for the rest of the study.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LLPS1-iLID::EGFP::FTH1 (pBS1042) was a gift from Pamela Silver (Addgene plasmid # 185289 ; http://n2t.net/addgene:185289 ; RRID:Addgene_185289)

  • For your References section:

    Natural and Designed Proteins Inspired by Extremotolerant Organisms Can Form Condensates and Attenuate Apoptosis in Human Cells. Veling MT, Nguyen DT, Thadani NN, Oster ME, Rollins NJ, Brock KP, Bethel NP, Lim S, Baker D, Way JC, Marks DS, Chang RL, Silver PA. ACS Synth Biol. 2022 Mar 18;11(3):1292-1302. doi: 10.1021/acssynbio.1c00572. Epub 2022 Feb 18. 10.1021/acssynbio.1c00572 PubMed 35176859
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