pST_APOE_HUMAN_E4_47-300 (pBS0847)
(Plasmid #185279)

• Purpose: For the mammalian expression of the human protein APOE_HUMAN_E4_47-300. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).
• Depositing Lab: Pamela Silver
• Publication: Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1302. doi: 10.1021/acssynbio.1c00572. Epub 2022 Feb 18.

Backbone

• Vector backbone: YK 02
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: APOE_HUMAN_E4_47-300
• Species: H. sapiens (human)
• Insert Size (bp): 768

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pBS0847.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is one of the top performing targets in our apoptosis assay (it protected from apoptosis). See supplemental table 2 in our paper to see its performance in our assay. The negative control for this assay is pSecTag2 B which can be obtained from Thermo here https://www.thermofisher.com/order/catalog/product/V90020