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Addgene

DAXX-myc-his
(Plasmid #1852)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1852 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1/myc-His
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DAXX
  • Alt name
    Fas death domain-associated protein
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2500
  • Entrez Gene
    Daxx
  • Tags / Fusion Proteins
    • myc (C terminal on backbone)
    • his (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 3′ cloning site EcoRV (destroyed during cloning)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See scanned map. NotI/BamHI will remove the insert.
(Leder #C-1156)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DAXX-myc-his was a gift from Philip Leder (Addgene plasmid # 1852 ; http://n2t.net/addgene:1852 ; RRID:Addgene_1852)

  • For your References section:

    Homeodomain-interacting protein kinase 1 modulates Daxx localization, phosphorylation, and transcriptional activity. Ecsedy JA, Michaelson JS, Leder P. Mol Cell Biol 2003 Feb;23(3):950-60. 10.1128/MCB.23.3.950-960.2003 PubMed 12529400
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