pG108-K - AhpC-Glow
(Plasmid #185135)

• Purpose: Bacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers BS2 is cloned under the Bacteroides thetaiotaomicron ahpC promoter
• Depositing Lab: Janina Lewis
• Publication: Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-191. doi: 10.1111/omi.12304. Epub 2020 Jul 9.

Backbone

• Vector backbone: pH108
• Backbone size w/o insert (bp): 700
• Vector type: Bacterial Expression ; pG106
• Selectable markers: kanamycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: Glow gene
• Alt name: BS2
• Species: Synthetic
• Insert Size (bp): 400

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: PstI (unknown if destroyed)
• 5′ sequencing primer: CTAGGATCCAAGAAGGCAGCTTCACTGGC
• 3′ sequencing primer: CGTACTGCAGTCACTCGAGCAGCTTTTCATA

Gene/Insert 2

• Gene/Insert name: Bacteroides thetaiotaomicron ahpC promoter
• Species: Synthetic
• Insert Size (bp): 300

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry