KBB491
(Plasmid
#185134)
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PurposeFull length SWI6 fused to activation domain of two-hybrid plasmid
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 185134 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepOAD
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSWI6
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SpeciesS. cerevisiae (budding yeast)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
KBB491 was a gift from Ken Belanger (Addgene plasmid # 185134 ; http://n2t.net/addgene:185134 ; RRID:Addgene_185134) -
For your References section:
Exportin Crm1 is important for Swi6 nuclear shuttling and MBF transcription activation in Saccharomyces cerevisiae. Belanger KD, Yewdell WT, Barber MF, Russo AN, Pettit MA, Damuth EK, Hussain N, Geier SJ, Belanger KG. BMC Mol Cell Biol. 2022 Feb 21;23(1):10. doi: 10.1186/s12860-022-00409-6. 10.1186/s12860-022-00409-6 PubMed 35189816