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Addgene

KBB553
(Plasmid #185107)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185107 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS315
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    POM152
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused to GFP with Y180L, Q182L
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    KBB553 was a gift from Ken Belanger (Addgene plasmid # 185107 ; http://n2t.net/addgene:185107 ; RRID:Addgene_185107)

  • For your References section:

    Characterization of nuclear pore complex targeting domains in Pom152 in Saccharomyces cerevisiae. Brown JT, Haraczy AJ, Wilhelm CM, Belanger KD. Biol Open. 2021 Oct 15;10(10). pii: 272274. doi: 10.1242/bio.057661. Epub 2021 Oct 20. 10.1242/bio.057661 PubMed 34557894
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