Skip to main content
Addgene

DAXX-myc
(Plasmid #1851)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1851 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCAGGS
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DAXX
  • Alt name
    Fas death domain-associated protein
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2500
  • Entrez Gene
    Daxx
  • Tag / Fusion Protein
    • myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (destroyed during cloning)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer pCAG-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Amino acids 1-739 of DAXX (entire ORF) PCR'd with myc tag at 3' end, cut with EcoRV, and cloned into blunted XhoI sites of pCAGGS. To be used for eukaryotic expression. Construct has ATG and Kozak.
(Leder #C-1155)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DAXX-myc was a gift from Philip Leder (Addgene plasmid # 1851 ; http://n2t.net/addgene:1851 ; RRID:Addgene_1851)

  • For your References section:

    Homeodomain-interacting protein kinase 1 modulates Daxx localization, phosphorylation, and transcriptional activity. Ecsedy JA, Michaelson JS, Leder P. Mol Cell Biol 2003 Feb;23(3):950-60. 10.1128/MCB.23.3.950-960.2003 PubMed 12529400
Related items:
Commonly requested with: