Skip to main content
Addgene

KBB382
(Plasmid #185085)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185085 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pRS315
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    POM152
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    Pom152 N280A, N398A, N???A, N???A = Pom152Dglyc
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    KBB382 was a gift from Ken Belanger (Addgene plasmid # 185085 ; http://n2t.net/addgene:185085 ; RRID:Addgene_185085)

  • For your References section:

    Nuclear pore complex function in Saccharomyces cerevisiae is influenced by glycosylation of the transmembrane nucleoporin Pom152p. Belanger KD, Gupta A, MacDonald KM, Ott CM, Hodge CA, Cole CM, Davis LI. Genetics. 2005 Nov;171(3):935-47. doi: 10.1534/genetics.104.036319. Epub 2005 Aug 22. 10.1534/genetics.104.036319 PubMed 16118201
Related items: