Skip to main content
Addgene

KBB364
(Plasmid #185083)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185083 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pRS316
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RFA1
  • Species
    S. cerevisiae (budding yeast)
  • Promoter GAL1
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    KBB364 was a gift from Ken Belanger (Addgene plasmid # 185083 ; http://n2t.net/addgene:185083 ; RRID:Addgene_185083)

  • For your References section:

    The karyopherin Kap95 and the C-termini of Rfa1, Rfa2, and Rfa3 are necessary for efficient nuclear import of functional RPA complex proteins in Saccharomyces cerevisiae. Belanger KD, Griffith AL, Baker HL, Hansen JN, Kovacs LA, Seconi JS, Strine AC. DNA Cell Biol. 2011 Sep;30(9):641-51. doi: 10.1089/dna.2010.1071. Epub 2011 Feb 20. 10.1089/dna.2010.1071 PubMed 21332387
Related items:
Commonly requested with: