KBB329
(Plasmid
#185077)
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PurposeNUP82 expression in yeast
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 185077 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS316
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNUP82
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SpeciesS. cerevisiae (budding yeast)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
KBB329 was a gift from Ken Belanger (Addgene plasmid # 185077 ; http://n2t.net/addgene:185077 ; RRID:Addgene_185077) -
For your References section:
The karyopherin Msn5/Kap142 requires Nup82 for nuclear export and performs a function distinct from translocation in RPA protein import. Belanger KD, Simmons LA, Roth JK, VanderPloeg KA, Lichten LB, Fahrenkrog B. J Biol Chem. 2004 Oct 15;279(42):43530-9. doi: 10.1074/jbc.M407641200. Epub 2004 Aug 4. 10.1074/jbc.M407641200 PubMed 15294903