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Addgene

KBB284
(Plasmid #185075)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185075 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS316
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RFA2
  • Species
    S. cerevisiae (budding yeast)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    KBB284 was a gift from Ken Belanger (Addgene plasmid # 185075 ; http://n2t.net/addgene:185075 ; RRID:Addgene_185075)

  • For your References section:

    The karyopherin Msn5/Kap142 requires Nup82 for nuclear export and performs a function distinct from translocation in RPA protein import. Belanger KD, Simmons LA, Roth JK, VanderPloeg KA, Lichten LB, Fahrenkrog B. J Biol Chem. 2004 Oct 15;279(42):43530-9. doi: 10.1074/jbc.M407641200. Epub 2004 Aug 4. 10.1074/jbc.M407641200 PubMed 15294903
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