KBB215
(Plasmid
#185068)
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PurposeSST::WAK2 EcoRI/ScaI fragment (secretion confirmed)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 185068 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneSST vector
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameWAK2
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SpeciesS. cerevisiae (budding yeast)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
KBB215 was a gift from Ken Belanger (Addgene plasmid # 185068 ; http://n2t.net/addgene:185068 ; RRID:Addgene_185068) -
For your References section:
A signal peptide secretion screen in Fucus distichus embryos reveals expression of glucanase, EGF domain-containing, and LRR receptor kinase-like polypeptides during asymmetric cell growth. Belanger KD, Wyman AJ, Sudol MN, Singla-Pareek SL, Quatrano RS. Planta. 2003 Oct;217(6):931-50. doi: 10.1007/s00425-003-1058-8. Epub 2003 Jun 27. 10.1007/s00425-003-1058-8 PubMed 12836024
