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Addgene

pRAR111
(Plasmid #185047)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185047 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ColE1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    L31p
  • Alt name
    ykgM
  • Species
    E. coli MG1655
  • Mutation
    Last 8 amino acids deleted of L31p coding region

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRAR111 was a gift from Julius Lucks (Addgene plasmid # 185047 ; http://n2t.net/addgene:185047 ; RRID:Addgene_185047)

  • For your References section:

    Zur and zinc increase expression of E. coli ribosomal protein L31 through RNA-mediated repression of the repressor L31p. Rasmussen RA, Wang S, Camarillo JM, Sosnowski V, Cho BK, Goo YA, Lucks JB, O'Halloran TV. Nucleic Acids Res. 2022 Dec 9;50(22):12739-12753. doi: 10.1093/nar/gkac1086. 10.1093/nar/gkac1086 PubMed 36533433
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