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Addgene

pRAR074-control-sfGFP
(Plasmid #185032)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185032 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p15A oripACYC
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    L31-sfGFP
  • Alt name
    rpmE-sfGFP
  • Species
    E. coli MG1655
  • Mutation
    Nucleotides of rpmE 5'UTR scrambled, Only first 90 nt of rpmE coding present

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRAR074-control-sfGFP was a gift from Julius Lucks (Addgene plasmid # 185032 ; http://n2t.net/addgene:185032 ; RRID:Addgene_185032)

  • For your References section:

    Zur and zinc increase expression of E. coli ribosomal protein L31 through RNA-mediated repression of the repressor L31p. Rasmussen RA, Wang S, Camarillo JM, Sosnowski V, Cho BK, Goo YA, Lucks JB, O'Halloran TV. Nucleic Acids Res. 2022 Dec 9;50(22):12739-12753. doi: 10.1093/nar/gkac1086. 10.1093/nar/gkac1086 PubMed 36533433
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