HIPK1-myc-his
(Plasmid
#1850)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 1850 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1/myc-His
- Backbone size w/o insert (bp) 5500
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHIPK1
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Alt nameHomeodomain-Interacting Protein Kinase 1
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SpeciesM. musculus (mouse)
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Entrez GeneHipk1 (a.k.a. Myak, 1110062K04Rik)
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Tags
/ Fusion Proteins
- myc (C terminal on backbone)
- his (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See scanned map.
(Leder #JE64)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HIPK1-myc-his was a gift from Philip Leder (Addgene plasmid # 1850 ; http://n2t.net/addgene:1850 ; RRID:Addgene_1850) -
For your References section:
Homeodomain-interacting protein kinase 1 modulates Daxx localization, phosphorylation, and transcriptional activity. Ecsedy JA, Michaelson JS, Leder P. Mol Cell Biol 2003 Feb;23(3):950-60. 10.1128/MCB.23.3.950-960.2003 PubMed 12529400
Map uploaded by the depositor.
