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pJT396 PB ITR and Cp36 attD pEF Puro-2a-tagBFP2 donor
(Plasmid #184952)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184952 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    n/a
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PuroR, P2A, mTagBFP2
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Donor DNA that is compatible with both PiggyBac transposase and with Cp36 recombinase

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJT396 PB ITR and Cp36 attD pEF Puro-2a-tagBFP2 donor was a gift from Michael Bassik & Ami Bhatt & Lacra Bintu & Patrick Hsu (Addgene plasmid # 184952 ; http://n2t.net/addgene:184952 ; RRID:Addgene_184952)

  • For your References section:

    Systematic discovery of recombinases for efficient integration of large DNA sequences into the human genome. Durrant MG, Fanton A, Tycko J, Hinks M, Chandrasekaran SS, Perry NT, Schaepe J, Du PP, Lotfy P, Bassik MC, Bintu L, Bhatt AS, Hsu PD. Nat Biotechnol. 2022 Oct 10. pii: 10.1038/s41587-022-01494-w. doi: 10.1038/s41587-022-01494-w. 10.1038/s41587-022-01494-w PubMed 36217031
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