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PurposeLentiviral delivery of recombinase landing pad
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 184949 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonen/a
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Vector typeMammalian Expression, Lentiviral, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKp03 Lenti pEF1a-attB LSR GFP
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Alt nameLarge serine recombinase from microbial mobile genetic element and GFP
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid can be used to integrate a landing pad for the recombinase into the genome. Then, a donor with a matching attachment site can be recombined into the landing pad. The attB Is downstream the pEF1a promoter, such that the donor can trap the promoter, which will also knockout expression of the recombinase and GFP.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJT306 Kp03 Lenti pEF-attB LSR GFP was a gift from Michael Bassik & Ami Bhatt & Lacra Bintu & Patrick Hsu (Addgene plasmid # 184949 ; http://n2t.net/addgene:184949 ; RRID:Addgene_184949) -
For your References section:
Systematic discovery of recombinases for efficient integration of large DNA sequences into the human genome. Durrant MG, Fanton A, Tycko J, Hinks M, Chandrasekaran SS, Perry NT, Schaepe J, Du PP, Lotfy P, Bassik MC, Bintu L, Bhatt AS, Hsu PD. Nat Biotechnol. 2022 Oct 10. pii: 10.1038/s41587-022-01494-w. doi: 10.1038/s41587-022-01494-w. 10.1038/s41587-022-01494-w PubMed 36217031