pJT303 Ec04 Lenti pEF-attB LSR GFP
(Plasmid #184946)

• Purpose: Lentiviral delivery of recombinase landing pad
• Depositing Labs: Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu
• Publication: Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.1038/s41587-022-01494-w. doi: 10.1038/s41587-022-01494-w.

Backbone

• Vector backbone: n/a
• Vector type: Mammalian Expression, Lentiviral, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ec04 Lenti pEF1a-attB LSR GFP
• Alt name: Large serine recombinase from microbial mobile genetic element and GFP
• Species: Synthetic

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pJT303 Ec04 Lenti pEF-attB LSR GFP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid can be used to integrate a landing pad for the recombinase into the genome. Then, a donor with a matching attachment site can be recombined into the landing pad. The attB Is downstream the pEF1a promoter, such that the donor can trap the promoter, which will also knockout expression of the recombinase and GFP.