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pCytERM-mTagBFP2
(Plasmid #184940)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184940 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pN1
  • Backbone size w/o insert (bp) 3948
  • Total vector size (bp) 4788
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CytERM-mTagBFP2
  • Species
    Synthetic
  • Insert Size (bp)
    840
  • GenBank ID
    NM_001171120.1
  • Promoter CMV
  • Tag / Fusion Protein
    • mTagBFP2 (C terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGGGCCCGGGATCCACCGGTCgccaccatgagcgagctgattaagg
  • 3′ sequencing primer gattatgatctagagtcgcggccgcttaattaagcttgtgccccagtttgctaggg
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCytERM-mTagBFP2 was a gift from Kiryl Piatkevich (Addgene plasmid # 184940 ; http://n2t.net/addgene:184940 ; RRID:Addgene_184940)

  • For your References section:

    Dual-expression system for blue fluorescent protein optimization. Papadaki S, Wang X, Wang Y, Zhang H, Jia S, Liu S, Yang M, Zhang D, Jia JM, Koster RW, Namikawa K, Piatkevich KD. Sci Rep. 2022 Jun 17;12(1):10190. doi: 10.1038/s41598-022-13214-0. 10.1038/s41598-022-13214-0 PubMed 35715437
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