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pSF11-wmTagBFP2
(Plasmid #184934)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184934 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSF11
  • Backbone size w/o insert (bp) 6093
  • Total vector size (bp) 6847
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mTagBFP2
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    754
  • Promoter tag-168

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ggaggacccttggctagcgtcgacggtaccaaaaATGTCTGAACTTATCAAGG
  • 3′ sequencing primer gatgacagcggccgatgcggagctcTTAGTTGAGCTTGTGTCC
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSF11-wmTagBFP2 was a gift from Kiryl Piatkevich (Addgene plasmid # 184934 ; http://n2t.net/addgene:184934 ; RRID:Addgene_184934)

  • For your References section:

    Dual-expression system for blue fluorescent protein optimization. Papadaki S, Wang X, Wang Y, Zhang H, Jia S, Liu S, Yang M, Zhang D, Jia JM, Koster RW, Namikawa K, Piatkevich KD. Sci Rep. 2022 Jun 17;12(1):10190. doi: 10.1038/s41598-022-13214-0. 10.1038/s41598-022-13214-0 PubMed 35715437
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