pEasyG3_zeo
(Plasmid
#184917)
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PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_zeo recombines in vivo with a PCR product from pEasyG3_mic.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 184917 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
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Vector typeBacterial Expression, CRISPR
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Selectable markersZeocin ; zeoMX, zeocin resistance, yeast selection marker
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namegRNA scaffold
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gRNA/shRNA sequenceNNNNNNNNNNNNNNNNNNNN
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SpeciesStreptococcus pyogenes
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEasyG3_zeo was a gift from Jeferson Gross (Addgene plasmid # 184917 ; http://n2t.net/addgene:184917 ; RRID:Addgene_184917) -
For your References section:
EasyGuide Plasmids Support in Vivo Assembly of gRNAs for CRISPR/Cas9 Applications in Saccharomyces cerevisiae. Jacobus AP, Barreto JA, de Bem LS, Menegon YA, Fier I, Bueno JGR, Dos Santos LV, Gross J. ACS Synth Biol. 2022 Nov 18;11(11):3886-3891. doi: 10.1021/acssynbio.2c00348. Epub 2022 Oct 18. 10.1021/acssynbio.2c00348 PubMed 36257021