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Addgene

pEasyG1_hph
(Plasmid #184912)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184912 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Bacterial Expression, Yeast Expression, CRISPR
  • Selectable markers
    Hygromycin ; hphMX, hygromycin resistance, yeast selection marker

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Alternative kanamycin selection marker
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gRNA scaffold
  • gRNA/shRNA sequence
    NNNNNNNNNNNNNNNNNNNN
  • Species
    Streptococcus pyogenes

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEasyG1_hph was a gift from Jeferson Gross (Addgene plasmid # 184912 ; http://n2t.net/addgene:184912 ; RRID:Addgene_184912)

  • For your References section:

    EasyGuide Plasmids Support in Vivo Assembly of gRNAs for CRISPR/Cas9 Applications in Saccharomyces cerevisiae. Jacobus AP, Barreto JA, de Bem LS, Menegon YA, Fier I, Bueno JGR, Dos Santos LV, Gross J. ACS Synth Biol. 2022 Nov 18;11(11):3886-3891. doi: 10.1021/acssynbio.2c00348. Epub 2022 Oct 18. 10.1021/acssynbio.2c00348 PubMed 36257021
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