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Addgene

pAFi-dHdrA
(Plasmid #184907)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184907 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBBRMCS2
  • Modifications to backbone
    changed kanamycin to chloramphenicol; inserted sgRNA and dCas9
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Cm-sgRNA-dCas9
  • Species
    Synthetic; streptococcus pyogenes
  • Insert Size (bp)
    5300

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr Yilan Liu from University of Toronto

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAFi-dHdrA was a gift from Radhakrishnan Mahadevan (Addgene plasmid # 184907 ; http://n2t.net/addgene:184907 ; RRID:Addgene_184907)

  • For your References section:

    Genetic Engineering of Acidithiobacillus ferridurans Using CRISPR Systems To Mitigate Toxic Release in Biomining. Chen J, Liu Y, Mahadevan R. Environ Sci Technol. 2023 Aug 22;57(33):12315-12324. doi: 10.1021/acs.est.3c02492. Epub 2023 Aug 9. 10.1021/acs.est.3c02492 PubMed 37556825
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