pAFi-dHdrA
(Plasmid
#184907)
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Purposecarrying CRISPR/dCas9 system for gene knockdown in Acidithiobacillus ferridurans
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 184907 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBBRMCS2
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Modifications to backbonechanged kanamycin to chloramphenicol; inserted sgRNA and dCas9
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Vector typeBacterial Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCm-sgRNA-dCas9
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SpeciesSynthetic; streptococcus pyogenes
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Insert Size (bp)5300
Resource Information
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A portion of this plasmid was derived from a plasmid made byDr Yilan Liu from University of Toronto
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.03.14.484339v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAFi-dHdrA was a gift from Radhakrishnan Mahadevan (Addgene plasmid # 184907 ; http://n2t.net/addgene:184907 ; RRID:Addgene_184907) -
For your References section:
Genetic Engineering of Acidithiobacillus ferridurans Using CRISPR Systems To Mitigate Toxic Release in Biomining. Chen J, Liu Y, Mahadevan R. Environ Sci Technol. 2023 Aug 22;57(33):12315-12324. doi: 10.1021/acs.est.3c02492. Epub 2023 Aug 9. 10.1021/acs.est.3c02492 PubMed 37556825
Map uploaded by the depositor.
