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Addgene

pMRX-IP-GFP-LC3-mRuby3
(Plasmid #184898)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184898 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMRX-IP
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LC3B
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    357
  • Tags / Fusion Proteins
    • EGFP (N terminal on insert)
    • mRuby3 (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMRX-IP-GFP-LC3-mRuby3 was a gift from Noboru Mizushima (Addgene plasmid # 184898 ; http://n2t.net/addgene:184898 ; RRID:Addgene_184898)

  • For your References section:

    Evolution and insights into the structure and function of the DedA superfamily containing TMEM41B and VMP1. Okawa F, Hama Y, Zhang S, Morishita H, Yamamoto H, Levine TP, Mizushima N. J Cell Sci. 2021 Apr 15;134(8). pii: 237813. doi: 10.1242/jcs.255877. Epub 2021 Apr 28. 10.1242/jcs.255877 PubMed 33771928
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