pMRX-IZ-ANXA1-mRuby3
(Plasmid
#184888)
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PurposeStable expression of ANXA1-mRuby3 (Annexin A1-mRuby3) in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 184888 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMRX-IZ
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Vector typeMammalian Expression
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameANXA1
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Alt nameAnnexin A1
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SpeciesH. sapiens (human)
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Insert Size (bp)1038
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Tag
/ Fusion Protein
- mRuby3 (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMRX-IZ-ANXA1-mRuby3 was a gift from Noboru Mizushima (Addgene plasmid # 184888 ; http://n2t.net/addgene:184888 ; RRID:Addgene_184888) -
For your References section:
Annexins A1 and A2 are recruited to larger lysosomal injuries independently of ESCRTs to promote repair. Yim WW, Yamamoto H, Mizushima N. FEBS Lett. 2022 Mar 11. doi: 10.1002/1873-3468.14329. 10.1002/1873-3468.14329 PubMed 35274304