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pCPF101-pfQC-cyclase dead
(Plasmid #184878)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184878 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCFP101
  • Backbone size w/o insert (bp) 5374
  • Modifications to backbone
    insert
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use BL21(DE3) Rosetta2 for protein expression.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pfQC-cyclase dead
  • Species
    P. Falciparum
  • Insert Size (bp)
    1067
  • Mutation
    F77A & Q79A
  • Tag / Fusion Protein
    • His tag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCPF101-pfQC-cyclase dead was a gift from Ferenc Scheeren (Addgene plasmid # 184878 ; http://n2t.net/addgene:184878 ; RRID:Addgene_184878)

  • For your References section:

    Malaria parasite evades mosquito immunity by glutaminyl cyclase-mediated posttranslational protein modification. Kolli SK, Molina-Cruz A, Araki T, Geurten FJA, Ramesar J, Chevalley-Maurel S, Kroeze HJ, Bezemer S, de Korne C, Withers R, Raytselis N, El Hebieshy AF, Kim RQ, Child MA, Kakuta S, Hisaeda H, Kobayashi H, Annoura T, Hensbergen PJ, Franke-Fayard BM, Barillas-Mury C, Scheeren FA, Janse CJ. Proc Natl Acad Sci U S A. 2022 Aug 30;119(35):e2209729119. doi: 10.1073/pnas.2209729119. Epub 2022 Aug 22. 10.1073/pnas.2209729119 PubMed 35994647
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