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pTOPO-col10a1-KI-donor
(Plasmid #184874)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184874 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR-bluntII-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3519
  • Total vector size (bp) 8217
  • Vector type
    Cre/Lox, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Collagen10a1 5' homology arm
  • Species
    Oryzias latipes
  • Insert Size (bp)
    292
  • GenBank ID
    NM_001201509.1

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GTAAAACGACGGCCAG
  • 3′ sequencing primer GGCGATCCCTGAACATGTCCAT
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Collagen10a1 3' homology arm
  • Species
    Oryzias latipes
  • Insert Size (bp)
    215
  • GenBank ID
    NM_001201509.1

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CAACTGATTTAAGCTTT
  • 3′ sequencing primer GTCATAGCTGTTTCCTG
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    p2a-CreERT2
  • Species
    Synthetic
  • Insert Size (bp)
    2040

Cloning Information for Gene/Insert 3

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ACAACAAAGGCTTCCTGGAC
  • 3′ sequencing primer CTAGTAACGGCCGCCAGTGT
    • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    Myosin light chain 2 promoter
  • Alt name
    cmlc2 promoter
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    899

Cloning Information for Gene/Insert 4

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GTCGCCGTCCAGCTCGACCA
  • 3′ sequencing primer TCTGTTGAGCACCTTTTTCTTG
    • (Common Sequencing Primers)

Gene/Insert 5

  • Gene/Insert name
    eGFP
  • Species
    Synthetic
  • Insert Size (bp)
    718

Cloning Information for Gene/Insert 5

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TCTGGATCTACGTAATACGACTCA
  • 3′ sequencing primer TGCTGGAATCTGAGCACTTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTOPO-col10a1-KI-donor was a gift from Christoph Winkler (Addgene plasmid # 184874 ; http://n2t.net/addgene:184874 ; RRID:Addgene_184874)

  • For your References section:

    A novel non-disruptive and efficient knock-in allows fate tracing of resident osteoblast progenitors during repair of vertebral lesions in medaka. Hui TW, Winkler C. Development. 2022 May 20. pii: 275483. doi: 10.1242/dev.200238. 10.1242/dev.200238 PubMed 35593425
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