TOPO-nts
(Plasmid
#184823)
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PurposeUsed to synthesize RNA probe for in situ hybridization for nts
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 184823 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCRII-TOPO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 4430
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Vector typetemplate for synthesis of nts probe
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert naments
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)428
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Entrez Genents (a.k.a. si:ch73-193m8.1)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer M13R
- 3′ sequencing primer M13F (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.04.07.487414v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TOPO-nts was a gift from Marnie Halpern (Addgene plasmid # 184823 ; http://n2t.net/addgene:184823 ; RRID:Addgene_184823) -
For your References section:
Genetically defined nucleus incertus neurons differ in connectivity and function. Spikol ED, Cheng J, Macurak M, Subedi A, Halpern ME. eLife 12:RP89516 10.7554/eLife.89516.2
