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Addgene

TOPO-nts
(Plasmid #184823)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184823 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCRII-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 4430
  • Vector type
    template for synthesis of nts probe

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    nts
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    428
  • Entrez Gene
    nts (a.k.a. si:ch73-193m8.1)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TOPO-nts was a gift from Marnie Halpern (Addgene plasmid # 184823 ; http://n2t.net/addgene:184823 ; RRID:Addgene_184823)

  • For your References section:

    Genetically defined nucleus incertus neurons differ in connectivity and function. Spikol ED, Cheng J, Macurak M, Subedi A, Halpern ME. eLife 12:RP89516 10.7554/eLife.89516.2
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