pPRISM-QF2-he1.1:YFP-rln3a-HA
(Plasmid #184820)

• Purpose: Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Contains homology arm inserts for integration of QF2 into rln3a gene by CRISPR/Cas9 integration.
• Depositing Lab: Marnie Halpern
• Publication: Spikol et al eLife 12:RP89516

Backbone

• Vector backbone: pPRISM
• Backbone size w/o insert (bp): 7395
• Total vector size (bp): 7499
• Vector type: CRISPR ; For integration of QF2 by homology-directed repair

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: rln3a-HA
• Species: D. rerio (zebrafish)
• Insert Size (bp): 104
• GenBank ID: NM_001037803.1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BfuAI (unknown if destroyed)
• 3′ cloning site: BspQI (unknown if destroyed)
• 5′ sequencing primer: GAG TCA GTG AGC GAG GAA GC
• 3′ sequencing primer: ACG TTT CCC GTT GAA TAT GGC TC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968).
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2022.04.07.487414v1 for bioRxiv preprint.