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pPRISM-QF2-he1.1:YFP
(Plasmid #184819)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184819 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPRISM
  • Backbone size w/o insert (bp) 7395
  • Vector type
    CRISPR ; Backbone for cloning of homology arms (for integration of QF2 by homology directed repair)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    QF2-pA-he1.1:YFP

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968).
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPRISM-QF2-he1.1:YFP was a gift from Marnie Halpern (Addgene plasmid # 184819 ; http://n2t.net/addgene:184819 ; RRID:Addgene_184819)

  • For your References section:

    Genetically defined nucleus incertus neurons differ in connectivity and function. Spikol ED, Cheng J, Macurak M, Subedi A, Halpern ME. eLife 12:RP89516 10.7554/eLife.89516.2
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