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Addgene

pDR274-gsc2-sgRNA
(Plasmid #184818)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184818 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDR274
  • Backbone size w/o insert (bp) 2147
  • Total vector size (bp) 2167
  • Vector type
    CRISPR ; template for sgRNA synthesis

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    gsc2-sgRNA
  • gRNA/shRNA sequence
    gsc2
  • Species
    D. rerio (zebrafish)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (unknown if destroyed)
  • 3′ cloning site BsaI (unknown if destroyed)
  • 5′ sequencing primer M13F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDR274-gsc2-sgRNA was a gift from Marnie Halpern (Addgene plasmid # 184818 ; http://n2t.net/addgene:184818 ; RRID:Addgene_184818)

  • For your References section:

    Genetically defined nucleus incertus neurons differ in connectivity and function. Spikol ED, Cheng J, Macurak M, Subedi A, Halpern ME. eLife 12:RP89516 10.7554/eLife.89516.2
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