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Addgene

pST44-AP-1 core
(Plasmid #184809)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184809 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pST44
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Hs AP1S3; Hs AP1B1 (1-584); Mm AP1G1 (1-595); Mm AP1M1
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Mutation
    AP1S3: S148C; AP1B1: K359R, E476K
  • Promoter T7
  • Tags / Fusion Proteins
    • His6 (N terminal on insert)
    • GST tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

AP1S3 in cassette1; 6His-TEVsite-AP1B1 (1-584) in cassette2; AP1G1 (1-595)-TEVsite-GST in cassette3; Mm AP1M1 in cassette4

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pST44-AP-1 core was a gift from James Hurley (Addgene plasmid # 184809 ; http://n2t.net/addgene:184809 ; RRID:Addgene_184809)

  • For your References section:

    Structural basis for recruitment and activation of the AP-1 clathrin adaptor complex by Arf1. Ren X, Farias GG, Canagarajah BJ, Bonifacino JS, Hurley JH. Cell. 2013 Feb 14;152(4):755-67. doi: 10.1016/j.cell.2012.12.042. 10.1016/j.cell.2012.12.042 PubMed 23415225
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