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Addgene

pST39-Ubc13-MMS2
(Plasmid #184807)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184807 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pST39
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Ubc13, MMS2
  • Species
    S. cerevisiae (budding yeast)
  • Promoter T7
  • Tag / Fusion Protein
    • His6 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Ubc13 in cassette 1; 6His-TEVsite-MMS2 in cassette2

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pST39-Ubc13-MMS2 was a gift from James Hurley (Addgene plasmid # 184807 ; http://n2t.net/addgene:184807 ; RRID:Addgene_184807)

  • For your References section:

    VHS domains of ESCRT-0 cooperate in high-avidity binding to polyubiquitinated cargo. Ren X, Hurley JH. EMBO J. 2010 Mar 17;29(6):1045-54. Epub 2010 Feb 11. 10.1038/emboj.2010.6 PubMed 20150893
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