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His-GST-Cys-M87 Spastin
(Plasmid #184804)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184804 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    2GT
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    M87 Spastin
  • Species
    H. sapiens (human)
  • Mutation
    A2C
  • Entrez Gene
    SPAST (a.k.a. ADPSP, FSP2, SPG4)
  • Promoter T7
  • Tag / Fusion Protein
    • His6-GST (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

6His-GST-TEVsite-Spastin (88-616-A2C)-stop, codon-optimized gene.

Please visit https://www.biorxiv.org/content/10.1101/2022.02.03.479062v2 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His-GST-Cys-M87 Spastin was a gift from James Hurley (Addgene plasmid # 184804 ; http://n2t.net/addgene:184804 ; RRID:Addgene_184804)

  • For your References section:

    Friction-driven membrane scission by the human ESCRT-III proteins CHMP1B and IST1. Cada AK, Pavlin MR, Castillo JP, Tong AB, Larsen KP, Ren X, Yokom AL, Tsai FC, Shiah JV, Bassereau PM, Bustamante CJ, Hurley JH. Proc Natl Acad Sci U S A. 2022 Jul 19;119(29):e2204536119. doi: 10.1073/pnas.2204536119. Epub 2022 Jul 11. 10.1073/pnas.2204536119 PubMed 35858336
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