pScalps ACE2 hygro
(Plasmid
#184786)
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PurposeStable expression of Human Ace2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 184786 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepScalps hygro
- Backbone size w/o insert (bp) 8184
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Vector typeLentiviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHuman ACE2
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SpeciesH. sapiens (human)
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Insert Size (bp)2430
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Entrez GeneACE2 (a.k.a. ACEH)
- Promoter SFFV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site HpaI (destroyed during cloning)
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pScalps ACE2 hygro was a gift from Ajit Chande (Addgene plasmid # 184786 ; http://n2t.net/addgene:184786 ; RRID:Addgene_184786) -
For your References section:
SARS CoV-2 Nucleoprotein Enhances the Infectivity of Lentiviral Spike Particles. Mishra T, Sreepadmanabh M, Ramdas P, Sahu AK, Kumar A, Chande A. Front Cell Infect Microbiol. 2021 Apr 23;11:663688. doi: 10.3389/fcimb.2021.663688. eCollection 2021. 10.3389/fcimb.2021.663688 PubMed 33968806