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Addgene

pScalps ACE2 hygro
(Plasmid #184786)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184786 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pScalps hygro
  • Backbone size w/o insert (bp) 8184
  • Vector type
    Lentiviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Human ACE2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2430
  • Entrez Gene
    ACE2 (a.k.a. ACEH)
  • Promoter SFFV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site HpaI (destroyed during cloning)
  • 5′ sequencing primer Unknown
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pScalps ACE2 hygro was a gift from Ajit Chande (Addgene plasmid # 184786 ; http://n2t.net/addgene:184786 ; RRID:Addgene_184786)
  • For your References section:

    SARS CoV-2 Nucleoprotein Enhances the Infectivity of Lentiviral Spike Particles. Mishra T, Sreepadmanabh M, Ramdas P, Sahu AK, Kumar A, Chande A. Front Cell Infect Microbiol. 2021 Apr 23;11:663688. doi: 10.3389/fcimb.2021.663688. eCollection 2021. 10.3389/fcimb.2021.663688 PubMed 33968806