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Addgene

FLT3-ITD-MSCV-IRES-GFP
(Plasmid #184779)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184779 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV-IRES-GFP
  • Backbone size w/o insert (bp) 6559
  • Total vector size (bp) 9562
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLT3
  • Alt name
    CD135
  • Alt name
    FLK2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3003
  • Mutation
    Activating internal tandem duplication (ITD) added (TCAGAGAATATGAATATGATC)
  • GenBank ID
  • Entrez Gene
    FLT3 (a.k.a. CD135, FLK-2, FLK2, STK1)
  • Promoter MSCV

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer CCCTTGAACCTCCTCGTTCGACC
  • 3′ sequencing primer GCATTCCTTTGGCGAGAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLT3-ITD-MSCV-IRES-GFP was a gift from Timothy Ley (Addgene plasmid # 184779 ; http://n2t.net/addgene:184779 ; RRID:Addgene_184779)
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