UG75-PAH1-3HA
(Plasmid
#184761)
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PurposePAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 184761 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneUG75
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePAH1
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SpeciesS. cerevisiae (budding yeast)
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Entrez GenePAH1 (a.k.a. YMR165C, SMP2)
- Promoter PAH1
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Tag
/ Fusion Protein
- 3HA (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
UG75-PAH1-3HA was a gift from Zhiping Xie (Addgene plasmid # 184761 ; http://n2t.net/addgene:184761 ; RRID:Addgene_184761) -
For your References section:
Excess diacylglycerol at the endoplasmic reticulum disrupts endomembrane homeostasis and autophagy. Li D, Yang SG, He CW, Zhang ZT, Liang Y, Li H, Zhu J, Su X, Gong Q, Xie Z. BMC Biol. 2020 Aug 28;18(1):107. doi: 10.1186/s12915-020-00837-w. 10.1186/s12915-020-00837-w PubMed 32859196