OM14-2EGFP-UG74-ter
(Plasmid
#184758)
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PurposeMarker for yeast mitochondria. Integration of 2xGFP tag at OM14 C terminus. Uses antibiotic resistance marker natMX6.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 184758 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneUG74-2GFP
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOM14
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneOM14 (a.k.a. YBR230C)
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Tag
/ Fusion Protein
- 2EGFP (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
OM14-2EGFP-UG74-ter was a gift from Zhiping Xie (Addgene plasmid # 184758 ; http://n2t.net/addgene:184758 ; RRID:Addgene_184758) -
For your References section:
Excess diacylglycerol at the endoplasmic reticulum disrupts endomembrane homeostasis and autophagy. Li D, Yang SG, He CW, Zhang ZT, Liang Y, Li H, Zhu J, Su X, Gong Q, Xie Z. BMC Biol. 2020 Aug 28;18(1):107. doi: 10.1186/s12915-020-00837-w. 10.1186/s12915-020-00837-w PubMed 32859196