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Addgene

pYN2_1
(Plasmid #184757)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184757 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MoClo-YTK
  • Total vector size (bp) 10429
  • Vector type
    Bacterial Expression, Yeast Expression, Synthetic Biology
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    Cas9
  • gRNA/shRNA sequence
    NA
  • Species
    Streptococcus pyogenes
  • Promoter PGK1
  • Tag / Fusion Protein
    • 2xNLS

Cloning Information for Gene/Insert 1

  • Cloning method Gateway Cloning
  • 5′ sequencing primer Golden Gate Assembly
  • 3′ sequencing primer Golden Gate Assembly
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    sgRNA scaffold where sfGFP is replaced with gRNA protospacer of interest, which will be proceeded by the HDV Ribozyme
  • gRNA/shRNA sequence
    NA-sgRNA scaffold
  • Species
    Synthetic
  • Promoter tRNA-Phe-HDV Ribozyme

Cloning Information for Gene/Insert 2

  • Cloning method Gateway Cloning
  • 5′ sequencing primer Golden Gate Assembly
  • 3′ sequencing primer Golden Gate Assembly
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    All parts derived from MoClo-YTK (Kit #1000000061)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYN2_1 was a gift from Yvonne Nygård (Addgene plasmid # 184757 ; http://n2t.net/addgene:184757 ; RRID:Addgene_184757)

  • For your References section:

    A CRISPR activation and interference toolkit for industrial Saccharomyces cerevisiae strain KE6-12. Camara E, Lenitz I, Nygard Y. Sci Rep. 2020 Sep 3;10(1):14605. doi: 10.1038/s41598-020-71648-w. 10.1038/s41598-020-71648-w PubMed 32884066
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