pcDNA-miR-21 toehold switch
(Plasmid
#184756)
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PurposeExpresses miR-21 sensor with RFP output
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 184756 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA 3.0
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRed Fluorescence Protein
- Promoter CMV
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Tag
/ Fusion Protein
- miR-21 toehold switch (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA-miR-21 toehold switch was a gift from Allen Liu (Addgene plasmid # 184756 ; http://n2t.net/addgene:184756 ; RRID:Addgene_184756) -
For your References section:
A Novel Synthetic Toehold Switch for MicroRNA Detection in Mammalian Cells. Wang S, Emery NJ, Liu AP. ACS Synth Biol. 2019 May 17;8(5):1079-1088. doi: 10.1021/acssynbio.8b00530. Epub 2019 May 6. 10.1021/acssynbio.8b00530 PubMed 31039307
